Abstract
We have isolated the aph(3")-Ic gene, encoding an aminoglycoside 3"-O-phosphotransferase [APH(3")-Ic], from a genomic library of an environmental Mycobacterium fortuitum strain, selecting for streptomycin resistance. APH(3")-Ic phosphorylates and inactivates streptomycin. Similar genes have been described in Streptomyces griseus and plasmid RSF1010. It is also present in some M. fortuitum clinical isolates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology*
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Escherichia coli Proteins / genetics
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Molecular Sequence Data
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Mycobacterium bovis / drug effects
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Mycobacterium fortuitum / drug effects*
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Mycobacterium fortuitum / genetics*
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Mycobacterium smegmatis / drug effects
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Plasmids / genetics
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Streptomycin / pharmacology*
Substances
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Anti-Bacterial Agents
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Escherichia coli Proteins
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mraZ protein, E coli
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Streptomycin