Abstract
We previously showed that deletion of the cell surface molecule mCD24 resulted in an increased proliferation in adult subventricular zone (SVZ). Here, we report an increased PSA-NCAM+/TuJ1- population in the mCD24-/- in vivo SVZ as well as in vitro neurospheres. Isolated in vitro, these cells were able to generate neurospheres. Proliferation studies, using BrdU incorporation, showed an increased proliferation in P7 mCD24-/- SVZ and neurospheres. Using electron microscopy, the same cell types were identified in the in vivo SVZ as well as in vitro neurospheres from the WT and mCD24-/- mice. In mixed neurospheres, formed with WT and EGFP/KO cells (enhanced green fluorescent protein mCD24-/-), the WT environment was able to control the proliferation rate of the mCD24-/- cells, but was unable to regulate their differentiation. We concluded that mCD24 acts cell nonautonomously to regulate transit-amplifying cells proliferation and/or differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Antigens, CD / physiology*
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CD24 Antigen
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Cell Differentiation / physiology*
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Cell Lineage / physiology
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Cell Proliferation*
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Cells, Cultured
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Green Fluorescent Proteins
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Lateral Ventricles / growth & development*
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Lateral Ventricles / metabolism*
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Lateral Ventricles / ultrastructure
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Male
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Membrane Glycoproteins / physiology*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microscopy, Electron, Transmission
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Neural Cell Adhesion Molecule L1 / metabolism
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Neurons / metabolism
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Neurons / ultrastructure
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P-Selectin / metabolism
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Sialic Acids / metabolism
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Stem Cells / metabolism*
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Stem Cells / ultrastructure
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Tubulin / metabolism
Substances
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Antigens, CD
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CD24 Antigen
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Cd24a protein, mouse
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Membrane Glycoproteins
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Neural Cell Adhesion Molecule L1
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P-Selectin
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Sialic Acids
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Tubulin
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beta3 tubulin, mouse
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polysialyl neural cell adhesion molecule
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Green Fluorescent Proteins