Abstract
In this study we investigated the signaling pathways triggered by Tat in human monocyte to induce TNF-alpha. In monocytes, the calcium, the PKA, and the PKC pathways are highly implicated in the expression of cytokine genes. Thus, these three major signaling pathways were investigated. Our data show that (i) PKC and calcium pathways are required for TNF-alpha production, whereas the PKA pathway seems to be not involved; (ii) downstream from PKC, activation of NFkappaB is essential while ERK1/2 MAP kinases, even though activated by Tat, are not directly involved in the pathway signaling leading to TNF-alpha production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium / metabolism
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Cells, Cultured
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Enzyme-Linked Immunosorbent Assay
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Gene Products, tat / metabolism*
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HIV-1 / metabolism*
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Humans
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases / metabolism
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Monocytes / metabolism*
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NFATC Transcription Factors
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Protein Kinase C / metabolism
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Signal Transduction*
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Tumor Necrosis Factor-alpha / analysis
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Tumor Necrosis Factor-alpha / biosynthesis*
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, tat
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NFATC Transcription Factors
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NFATC3 protein, human
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Tumor Necrosis Factor-alpha
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tat Gene Products, Human Immunodeficiency Virus
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Protein Kinase C
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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Calcium