Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases

Laurent Poirel; Peter Kämpfer; Patrice Nordmann

doi:10.1128/AAC.46.12.4038-4040.2002

Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases

Antimicrob Agents Chemother. 2002 Dec;46(12):4038-40. doi: 10.1128/AAC.46.12.4038-4040.2002.

Authors

Laurent Poirel¹, Peter Kämpfer, Patrice Nordmann

Affiliation

¹ Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.

Abstract

A chromosome-encoded beta-lactamase gene, cloned and expressed in Escherichia coli from Kluyvera georgiana reference strain CUETM 4246-74 (DSM 9408), encoded the extended-spectrum beta-lactamase KLUG-1, which shared 99% amino acid identity with the plasmid-mediated beta-lactamase CTX-M-8. This work provides further evidence that Kluyvera spp. may be the progenitor(s) of CTX-M-type beta-lactamases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins*
Cloning, Molecular
Enterobacteriaceae / drug effects
Enterobacteriaceae / genetics*
Escherichia coli / genetics
Microbial Sensitivity Tests
Molecular Sequence Data
Sequence Homology, Amino Acid
beta-Lactamases / drug effects
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
blaCTX-M-8 protein, Citrobacter amalonaticus
beta-Lactamases

Associated data

GENBANK/AF501233