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Inhibition of full length C-terminal His/FLAG tagged human recombinant HDAC1 (1 to 482 residues) expressed in baculovirus-infected Sf9 cells using RHK-K(Ac)-AMC as substrate incubated for 2 hrs
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryRelated BioAssays by Target
Inhibition of HDAC in human HeLa nuclear extract assessed as residual activity at 20 uM relative to control
Assay data:16 Tested
Inhibition of HDAC in human HCT-116 cells assessed as increase in alpha tubulin acetylation at 1 uM incubated for 24 hrs by Western blot analysis
Assay data:1 Active, 1 Tested
Inhibition of HDAC in human HCT-116 cells assessed as increase in histone H3 acetylation at 1 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human HCT-116 cells assessed as increase in alpha tubulin acetylation incubated for 24 hrs by Western blot analysis
Assay data:1 Tested
Inhibition of HDAC in human HCT-116 cells assessed as increase in histone H3 acetylation incubated for 24 hrs by Western blot analysis
Inhibition of HDAC2 (unknown origin)
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of HDAC1 (unknown origin)
Inhibition of HDAC in human HeLa nuclear extract at 0.1 uM using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay
Assay data:9 Tested
Inhibition of HDAC2 (unknown origin) using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 1 hr by fluorescence assay
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
Inhibition of HDAC1 (unknown origin) using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 2 hrs by fluorescence assay
Inhibition of HDAC in human HeLa nuclear extract using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay
Assay data:11 Active, 11 Activity ≤ 1 µM, 11 Tested
Inhibition of HDAC in human HeLa nuclear extract at 0.1 uM using Boc-Lys(Ac)-AMC or Boc-Lys(Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay relative to control
Assay data:30 Tested
Inhibition of HDAC in human HepG2 cells assessed as increase in histone H3 acetylation at 5 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human Huh-7 cells assessed as increase in histone H3 acetylation at 5 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human HepG2 cells assessed as increase in histone H3 acetylation at 20 uM incubated for 24 hrs by Western blot analysis
Assay data:1 Active, 2 Tested
Inhibition of HDAC in human Huh-7 cells assessed as increase in histone H3 acetylation at 20 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human HepG2 cells assessed as increase in histone H3 acetylation at 20 to 40 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human Huh-7 cells assessed as increase in histone H3 acetylation at 20 to 40 uM incubated for 24 hrs by Western blot analysis
Inhibition of HDAC in human HeLa cell extracts using Boc-Lys(Ac)-AM as substrate by fluorescence based analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 5 Tested
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