Gsta2 glutathione S-transferase alpha 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gsta2provided by RGD
Official Full Name: glutathione S-transferase alpha 2provided by RGD
Primary source: RGD:2754
See related: Ensembl:ENSRNOG00000029861 AllianceGenome:RGD:2754
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Gsta1
Summary: Enables several functions, including dinitrosyl-iron complex binding activity; glutathione binding activity; and glutathione transferase activity. Involved in aging and xenobiotic catabolic process. Located in cytosol. Human ortholog(s) of this gene implicated in asthma and ovarian cancer. Orthologous to several human genes including GSTA2 (glutathione S-transferase alpha 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 4798.1), Kidney (RPKM 2325.2) and 1 other tissue See more
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Genomic context

Location:: 8q31

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (88064829..88077168, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (79184535..79196827, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (85640081..85645621, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1.
Title: Nephrotoxicity of hexachloro-1:3-butadiene in the male Hanover Wistar rat; correlation of minimal histopathological changes with biomarkers of renal injury.
C/EBP alpha and beta are involved in glucocorticoid-dependent repression of GSTA2 gene expression
Title: Regulation of the rat glutathione S-transferase A2 gene by glucocorticoids: crosstalk through C/EBPs.
a considerable amount of GSTs, in particular GSTA1-1, GSTA2-2, and GSTA3-3, corresponding to 10% of the cytosolic pool is electrostatically associated with the outer nuclear membrane, and a similar quantity is compartmentalized inside the nucleus
Title: Electrostatic association of glutathione transferase to the nuclear membrane. Evidence of an enzyme defense barrier at the nuclear envelope.
Oltipraz-induced GSTA2 gene expression is dependent upon PI3-kinase-mediated nuclear translocation and binding of C/EBP-beta to the GSTA2 gene promoter.
Title: Essential role of phosphatidylinositol 3-kinase-dependent CCAAT/enhancer binding protein beta activation in the induction of glutathione S-transferase by oltipraz.
P.893: Ceramide inhibits C/EBP-beta or Nrf2 activation, which contributes to repression of GSTA2 gene transactivation. P. 897: "...ceramide inhibition of... transcription factors contributes to the repression of the GSTA2 gene transactivation."
Title: Ceramide, an apoptotic rheostat, inhibits CCAAT/enhancer binding protein-beta and NF-E2-related factor-2 activation: the role in glutathione S-transferase A2 gene repression.
Mechanism of negative regulation of rat glutathione S-transferase A2 by the cytokine interleukin 6.
Title: Mechanism of negative regulation of rat glutathione S-transferase A2 by the cytokine interleukin 6.
dexamethasone antagonizes C/EBPbeta- and Nrf2-mediated GSTA2 gene induction via ligand-GR binding to the GRE, and steroid-mediated GSTA2 repression involves inactivation of C/EBPbeta and Nrf2 by SMRT recruited to steroid-GR complex
Title: Glucocorticoid receptor (GR)-associated SMRT binding to C/EBPbeta TAD and Nrf2 Neh4/5: role of SMRT recruited to GR in GSTA2 gene repression.
Single mutations are introduced to evaluate the importance of amino acids at the subunit interface of GSTA1-1. Phe52 and Arg69 are found to be major determinants of dimer formation: a single mutation at either position substantially hinders dimerization.
Title: Subunit interface residues of glutathione S-transferase A1-1 that are important in the monomer-dimer equilibrium.

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General gene information

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Markers

PMC133753P1 (e-PCR)
- UniSTS:270650

RH143684 (e-PCR)
- UniSTS:232930

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables dinitrosyl-iron complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables glutathione binding	IDA Inferred from Direct Assay more info	PubMed
enables glutathione transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutathione transferase activity	IEA Inferred from Electronic Annotation more info
enables glutathione transferase activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables steroid delta-isomerase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cellular oxidant detoxification	IEA Inferred from Electronic Annotation more info
involved_in epithelial cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in glutathione derivative biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glutathione metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in prostaglandin metabolic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in response to stilbenoid	ISO Inferred from Sequence Orthology more info
involved_in xenobiotic catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in xenobiotic metabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutathione S-transferase alpha-1

Names: 13-hydroperoxyoctadecadienoate peroxidase; GST 1-1; GST 1a-1a; GST A1-1; GST B; GST Ya1; Glutathione-S-transferase alpha type (Yc?); androst-5-ene-3,17-dione isomerase; glutathione S-transferase A2; glutathione S-transferase Ya subunit; glutathione S-transferase Ya-1; glutathione-S-transferase, alpha type2; ligandin

NP_058709.2

EC 2.5.1.18

XP_038936725.1

EC 2.5.1.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017013.4 → NP_058709.2 glutathione S-transferase alpha-1

See identical proteins and their annotated locations for NP_058709.2

Status: VALIDATED

Source sequence(s)

BC078706, K01931

UniProtKB/Swiss-Prot

P00502, P04903, Q63715, Q6AZ72

UniProtKB/TrEMBL

B6DYP7

Related

ENSRNOP00000037786.6, ENSRNOT00000032185.6

Conserved Domains (2) summary

cd03208
Location:86 → 220

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

cd03077
Location:4 → 82

GST_N_Alpha; GST_N family, Class Alpha subfamily; GSTs are cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

88064829..88077168 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039080797.2 → XP_038936725.1 glutathione S-transferase alpha-1 isoform X1

UniProtKB/Swiss-Prot

P00502, P04903, Q63715, Q6AZ72

UniProtKB/TrEMBL

B6DYP7

Conserved Domains (2) summary

cd03208
Location:86 → 220

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

cd03077
Location:4 → 82

GST_N_Alpha; GST_N family, Class Alpha subfamily; GSTs are cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, ...