U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    TKL2 transketolase TKL2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852414, updated on 9-Jun-2024

    Summary

    Go to the top of the page Help
    Gene symbol
    TKL2
    Gene description
    transketolase TKL2
    Primary source
    FungiDB:YBR117C
    Locus tag
    YBR117C
    See related
    SGD:S000000321; AllianceGenome:SGD:S000000321; VEuPathDB:YBR117C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Predicted to enable transketolase activity. Involved in pentose-phosphate shunt. Located in cytoplasm and nucleus. Orthologous to several human genes including TKT (transketolase) and TKTL2 (transketolase like 2). [provided by Alliance of Genome Resources, Apr 2022]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See TKL2 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (474392..476437, complement)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene DNA repair protein RAD16 Neighboring gene L-aminoadipate-semialdehyde dehydrogenase Neighboring gene translation elongation factor EF-1 alpha Neighboring gene Mud1p

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism. Blank HM, et al. Mol Biol Cell, 2020 May 1. PMID 32129706, Free PMC Article
    2. Analysis of a transketolase gene from Kluyveromyces lactis reveals that the yeast enzymes are more related to transketolases of prokaryotic origins than to those of higher eukaryotes. Jacoby JJ, et al. Curr Genet, 1997 Jan. PMID 9000376
    3. Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae. Sevostyanova IA, et al. Biochemistry (Mosc), 2009 Jul. PMID 19747100
    4. Theoretical model of interactions between ligand-binding sites in a dimeric protein and its application for the analysis of thiamine diphosphate binding to yeast transketolase. Ospanov R, et al. Biophys Chem, 2006 Nov 20. PMID 16837121
    5. Two methods for determination of transketolase activity. Sevostyanova IA, et al. Biochemistry (Mosc), 2006 May. PMID 16732737

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. characterization of NQM1 and TKL2, together with TAL1 and TKL1, regarding their roles in xylose utilization and fermentation
      Title: Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae.
    2. In the presence of Ca2+, the active sites of TK1 and TK2 exhibit negative cooperativity in binding both xylulose-5-phosphate and ribose-5-phosphate and positive cooperativity in the catalytic transformation of the substrates.
      Title: Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transketolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transketolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transketolase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in pentose-phosphate shunt IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pentose-phosphate shunt IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in purine nucleotide metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus HDA PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    transketolase TKL2
    NP_009675.3
    • Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      474392..476437 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178465.3 → NP_009675.3  TPA: transketolase TKL2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009675.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQB5, P33315
      UniProtKB/TrEMBL
      A0A6A5Q1W7, A6ZL62, B3LN38, B5VE78, C7GUL3, D3UEK9, G2W9C2, N1PAT1
      Conserved Domains (1) summary
      TIGR00232
      Location:9 → 665
      tktlase_bact; transketolase, bacterial and yeast
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P33315.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools