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Cyb5r3 cytochrome b5 reductase 3 [ Rattus norvegicus (Norway rat) ]

Gene ID: 25035, updated on 2-May-2024

Summary

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Official Symbol
Cyb5r3provided by RGD
Official Full Name
cytochrome b5 reductase 3provided by RGD
Primary source
RGD:2502
See related
Ensembl:ENSRNOG00000009592 AllianceGenome:RGD:2502
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Dia1; Nadhcb5; RNNADHCB5
Summary
Enables nucleotide binding activity. Predicted to be involved in nitric oxide biosynthetic process. Located in endoplasmic reticulum membrane. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Liver (RPKM 562.3), Lung (RPKM 375.6) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Cyb5r3 in Genome Data Viewer
Location:
7q34
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 7 NC_086025.1 (116186729..116204290, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 7 NC_051342.1 (114306686..114324247, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 7 NC_005106.4 (124024003..124041564, complement)

Chromosome 7 - NC_086025.1Genomic Context describing neighboring genes Neighboring gene transfer RNA proline (anticodon AGG) 38 Neighboring gene RNA, U12 small nuclear Neighboring gene alpha 1,4-galactosyltransferase Neighboring gene ADP-ribosylation factor GTPase activating protein 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. Samhan-Arias AK, et al. Brain Struct Funct, 2016 May. PMID 25850901
  2. The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site. Bewley MC, et al. Biochemistry, 2003 Nov 18. PMID 14609324
  3. The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent. Bewley MC, et al. Biochemistry, 2001 Nov 13. PMID 11695905
  4. Molecular basis of recessive congenital methemoglobinemia, types I and II: Exon skipping and three novel missense mutations in the NADH-cytochrome b5 reductase (diaphorase 1) gene. Kugler W, et al. Hum Mutat, 2001 Apr. PMID 11295830
  5. High-level expression in Escherichia coli of the soluble, catalytic domain of rat hepatic cytochrome b5 reductase. Barber MJ, et al. Protein Expr Purif, 1996 Aug. PMID 8812833

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. results highlight the importance of CYB5R3 and NQO1, two NAD(+) producers, for the promotion of health and extended lifespan
    Title: Overexpression of CYB5R3 and NQO1, two NAD(+) -producing enzymes, mimics aspects of caloric restriction.
  2. Results point out the enrichment of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in neuronal cells and structures of the cerebellum and brain stem.
    Title: High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.
  3. The data obtained argue against VDAC1 and in favor of Cyb5R3 involvement in the activation of redox cyclers in the outer mitochondrial membrane.
    Title: External mitochondrial NADH-dependent reductase of redox cyclers: VDAC1 or Cyb5R3?
  4. Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein
    Title: Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands.
  5. Structure of cytochrome b5 reductase S127P mutant reveals that the adenosine diphosphate (ADP) moiety of the FAD prosthetic group is displaced into the corresponding ADP binding site of the physiologic substrate NADH, thus acting as a substrate inhibitor.
    Title: The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ADP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables AMP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables FAD binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables FAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables FAD binding ISO
Inferred from Sequence Orthology
more info
  
enables NAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytochrome-b5 reductase activity, acting on NAD(P)H IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytochrome-b5 reductase activity, acting on NAD(P)H IEA
Inferred from Electronic Annotation
more info
  
enables cytochrome-b5 reductase activity, acting on NAD(P)H ISO
Inferred from Sequence Orthology
more info
  
enables cytochrome-b5 reductase activity, acting on NAD(P)H TAS
Traceable Author Statement
more info
 PubMed 
enables flavin adenine dinucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables flavin adenine dinucleotide binding ISO
Inferred from Sequence Orthology
more info
  
contributes_to nitrite reductase (NO-forming) activity ISO
Inferred from Sequence Orthology
more info
  
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in biological_process ND
No biological Data available
more info
  
involved_in cholesterol biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum membrane ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum membrane ISO
Inferred from Sequence Orthology
more info
  
located_in lipid droplet ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrial outer membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of nitric-oxide synthase complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
NADH-cytochrome b5 reductase 3
Names
B5R
Diaphorase (NADH) (cytochrome b-5 reductase)
diaphorase-1
NP_620232.1
XP_006242127.1
XP_038934366.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_138877.1 → NP_620232.1  NADH-cytochrome b5 reductase 3

    See identical proteins and their annotated locations for NP_620232.1

    Status: PROVISIONAL

    Source sequence(s)
    D00636
    UniProtKB/Swiss-Prot
    P20070, Q64569, Q64720
    UniProtKB/TrEMBL
    A0A8L2QWB9, A6HT85
    Related
    ENSRNOP00000077900.1, ENSRNOT00000102247.1
    Conserved Domains (2) summary
    cd06183
    Location:45 → 301
    cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
    PTZ00319
    Location:16 → 301
    PTZ00319; NADH-cytochrome B5 reductase; Provisional

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086025.1 Reference GRCr8

    Range
    116186729..116204290 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006242065.5 → XP_006242127.1  NADH-cytochrome b5 reductase 3 isoform X1

    UniProtKB/TrEMBL
    A0A8L2QWB9
    Related
    ENSRNOP00000082175.1
    Conserved Domains (2) summary
    cd06183
    Location:51 → 307
    cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...
    PTZ00319
    Location:22 → 307
    PTZ00319; NADH-cytochrome B5 reductase; Provisional

  2. XM_039078438.2 → XP_038934366.1  NADH-cytochrome b5 reductase 3 isoform X2

    UniProtKB/TrEMBL
    A0A8L2QWB9
    Conserved Domains (1) summary
    PLN02252
    Location:15 → 278
    PLN02252; nitrate reductase [NADPH]
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20070.2 GenPept UniProtKB/Swiss-Prot:P20070

Gene LinkOut

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