GEO DataSets

Analysis of spinal cord segment from interleukin (IL)-1α -/- and IL-1β -/- C57BL/6 adults 24 hrs after spinal cord injury (SCI). IL-1α and IL-1β belong to a family of cytokines playing a key role in neurodegeneration. Results provide insight into the role of IL-1α in the neuropathology of SCI.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation sets

Platform:

GPL6246

Series:

GSE70302

12 Samples

Download data: CEL

(Submitter supplied) We have previously shown that Il1a-knockout (KO) mice exhibit rapid (at day 1) and persistent improvements in locomotion associated with reduced lesion volume compared with Il1b-KO mice and C57BL/6 controls after traumatic spinal cord injury (SCI). To investigate the mechanism by which Il1a mediates its detrimental effect, we analyzed the transcriptome of the injured spinal cord of Il1a-KO, Il1b-KO and C57BL/6 mice at 24 hours after SCI using GeneChip microarrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5825

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Spinal cord injured mice were treated with IL-13, IL-4 or PBS. Populations of macrophages and microglia were sorted and analyzed separately.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: TXT

(Submitter supplied) Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Chd7 is ATP-dependent chromatin-remodeling enzyme. We performed microarray analysis to examine changes in gene expression between control and Chd7 knockdown OPCs. Results provide insight into the function of Chd7 in oligodendrocyte development.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL22438

6 Samples

Download data: TXT

(Submitter supplied) Next-generation humanized mouse models and single-cell RNA sequencing approaches enable in-depth studies into human immune cell biology. Here we used NSG-SGM3 mice engrafted with human umbilical cord haematopoietic stem cells to investigate how human immune cells respond to and/or are changed by traumatic spinal cord injury (SCI). Our behavioural studies firstly show that the benefits of immunocompromise on SCI recovery are lost with the introduction of human immune cells into these mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: MTX, TSV

(Submitter supplied) Infiltrating monocyte derived macrophages (MDMs) and resident microglia dominate CNS injury sites. We show that MDMs and microglia can directly communicate to modulate each other’s function. Also, the presence of MDMs in CNS injury suppresses microglia-mediated phagocytosis and inflammation. We suggest that macrophages infiltrating the injured CNS provide a mechanism to control acute and chronic microglia-mediated inflammation, which could otherwise drive damage in a variety of CNS conditions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

16 Samples

Download data: CEL, CHP

(Submitter supplied) Traumatic spinal cord injury (SCI) triggers a neuro-inflammatory response dominated by tissue-resident microglia and monocyte derived macrophages (MDMs). Since activated microglia and MDMs are morphologically identical and express similar phenotypic markers in vivo, identifying injury responses specifically coordinated by microglia has historically been challenging. Here, we pharmacologically depleted microglia and use anatomical, histopathological, tract tracing, bulk and single cell RNA sequencing to reveal the cellular and molecular responses to SCI controlled by microglia. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

20 Samples

Download data: CSV, MTX, TSV, XLSX

(Submitter supplied) To better understand which genes/pathways are activated in injured muscles following a spinal cord injury (SCI) and how these promote the development of neurogenic heterotopic ossification (NHO), we performed gene expression profiling by microarray of muscles in the early phase following muscle injury.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

16 Samples

Download data: IDAT, TXT

(Submitter supplied) Single-cell RNA sequencing of immune cells of mouse spinal cord using Drop-Seq

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: MTX, TSV

(Submitter supplied) OL translatome was determined in female RiboTag:Plp1-CreERT2 mice (C57Bl6 background) at 2, 10, and, 42 days after moderate contusive T9 SCI. Ribotag expression was induced by daily tamoxifen injections (1 mg i.p. over 8 days). Three weeks after completion of the induction protocol moderate contusive SCI was performed at T9 level (IH imactor, 50 kdyn). Uninjured mice (naive) were used as controls. At the time of surgery, animal age was 10-12 weeks. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

24 Samples

Download data: TXT

(Submitter supplied) Phospholipase D (PLD), a critical lipid signaling molecule, is known to be involved in various physiological processes. Despite this knowledge, the specific role of PLD in spinal cord injury remains unclear. In this study, we constructed mouse models of SCI and administered PLD inhibitor(FIPI) treatment to investigate the efficacy of PLD. Transcriptome sequencing analysis implicated inhibition of PLD altered gene expression in the inflammation regulation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: TXT

(Submitter supplied) Individuals with SCI are severely affected by immune system changes, resulting in a SCI-induced immune deficiency syndrome (SCI-IDS). While recent data support that acute SCI-IDS symptoms differ from those present in the chronic phase, only limited data in humans is available. To characterize dynamic molecular and cellular immune phenotypes over the first year, we assess RNA (bulk-RNA sequencing), protein, and flow cytometry (FACS) profiles of blood samples from 12 individuals with SCI at 0-3 days and at 3, 6, and 12 months post injury (MPI) compared to 23 uninjured individuals (controls). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

46 Samples

Download data: XLSX

(Submitter supplied) Oligodendrocytes are the primary target of demyelinating disorders in the CNS, which may evolve in neurodegeneration. DNA damage and oxidative stress are considered key pathogenic events, but the underlying molecular mechanisms remain unclear. Moreover, comprehensive animal models are lacking or do not fully recapitulate human diseases, complicating the path to effective treatments. Here we report that mice with cell autonomous deletion of the COP9 signalosome component CSN5 (Jab1) in oligodendrocytes develop DNA damage and defective DNA repair in myelinating glial cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: TXT

(Submitter supplied) Spinal cord injury (SCI) leads to severe sensory and motor dysfunction below the lesion. However, the cellular heterogeneity and dynamic responses in different regions below the lesion remain to be elusive. Here, we used single-cell transcriptomics to characterize the region-related cellular responses in complete thoracic SCI of rhesus monkeys from the acute to the chronic phase. We found that cells in the distal lumbar tissue were severely affected and generated a degenerative microenvironment dominated by sustained activation of phagocytic microglia, increased inhibitory interneurons, and demyelinated oligodendrocytes after SCI. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27943

20 Samples

Download data: MTX, TSV

(Submitter supplied) The lumbar spinal cord is fundamental for hindlimb function and serves as spared tissue after spinal cord injury (SCI). However, our knowledge about cellular heterogeneity of the intact spinal cord and spared tissues is still scarce especially in primates. Here, we used single-nucleus RNA sequencing analysis in rhesus monkey to characterize the cellular heterogeneity of the spinal cord and profile the dynamic changes of cells in spared tissues after complete thoracic SCI. more...

Organism:

Macaca mulatta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23949

7 Samples

Download data: MTX, TSV

(Submitter supplied) Interleukin-12 (IL-12) is a potent driver of type 1 immunity. Paradoxically, in autoimmune conditions, including of the CNS, IL-12 reduces inflammation. The underlying mechanism behind these opposing properties and the involved cellular players remain elusive. Here, we map IL-12 receptor (IL- 12R) expression to NK and T cells as well as neurons and oligodendrocytes. Conditionally ablating the IL-12R across these cell types in adult mice and assessing their susceptibility to experimental autoimmune encephalomyelitis revealed that the neuroprotective role of IL-12 is mediated by neuroectoderm-derived cells, specifically neurons, and not immune cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

11 Samples

Download data: MTX, TSV

(Submitter supplied) Interleukin-12 (IL-12) is a potent driver of type 1 immunity. Paradoxically, in autoimmune conditions, including of the CNS, IL-12 reduces inflammation. The underlying mechanism behind these opposing properties and the involved cellular players remain elusive. Here, we map IL-12 receptor (IL- 12R) expression to NK and T cells as well as neurons and oligodendrocytes. Conditionally ablating the IL-12R across these cell types in adult mice and assessing their susceptibility to experimental autoimmune encephalomyelitis revealed that the neuroprotective role of IL-12 is mediated by neuroectoderm-derived cells, specifically neurons, and not immune cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: TXT

(Submitter supplied) Traumatic spinal cord injury (SCI) is a debilitating central nervous system (CNS) pathology that lacks effective restorative treatments. The mechanisms of tissue recovery after SCI are dysregulated in comparison to normal wound healing, leading to a chronic wound state, rather than a return to homeostasis. While this state is characterized by persistent inflammation driven by CNS resident microglia (MG) and infiltrating myeloid cells, the precise roles of myeloid cell subsets after SCI remains unclear. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

25 Samples

Download data: CSV, TXT

(Submitter supplied) Both brain-resident microglia and peripheral macrophages are important cellular effectors of the inflammatory response to neural injury. They respond to neural injury by secreting a wide range of effector molecules including cytokines, chemokines and neurotrophic factors. To identify additional secreted signalling molecules, we used RNA-seq gene expression profiling to detect changes in the transcriptome of macrophage-lineage cells after acute neural injury in larval zebrafish. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24995

12 Samples

Download data: CSV