GEO DataSets

(Submitter supplied) Liver-specific ten-eleven translocation methylcytosine dioxygenases 2 and 3 (Tet2 plus Tet3)-deficient hepatitis B virus (HBV) transgenic mice fail to support viral biosynthesis. The levels of viral transcription and replication intermediates are dramatically reduced. Hepatitis B core antigen (HBcAg) is only observed in a very limited number of pericentral hepatocytes in a pattern that is similar to glutamate-ammonia ligase (Glul), a -catenin target gene. more...

Organism:

Hepatitis B virus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL33735

38 Samples

Download data: FA, TXT

(Submitter supplied) Infection-dependent transcriptional changes and the impact of antiviral therapy on viral replication can be measured in longitudinal human liver biopsies using single-cell RNA sequencing data

Organism:

Hepatitis B virus

Type:

Other

Platform:

GPL32478

12 Samples

Download data: BAM, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Hepatitis B virus; Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL33150 GPL24676 GPL30882

42 Samples

Download data: BEDGRAPH, TBI

(Submitter supplied) Chronic hepatitis B virus (HBV) infection is an incurable global health threat capable of causing liver disease and hepatocellular carcinoma. During the genesis of infection, HBV establishes an independent chromosome, cccDNA, consisting of the circular viral genome and host histones. The first viral protein expressed, HBx, induces degradation of a host silencing factor to facilitate infection. However, the relationship between cccDNA’s chromatin and early HBx transcription state remains poorly understood. more...

Organism:

Homo sapiens; Hepatitis B virus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL33150 GPL30882

27 Samples

Download data: BEDGRAPH, TBI

(Submitter supplied) We collected sequences amplified from three HBV-genome-encoding plasmids, one wild type and two with predefined point mutations.

Organism:

Hepatitis B virus; blank sample

Type:

Other

Platforms:

GPL30314 GPL32478

42 Samples

Download data: VCF

(Submitter supplied) HBV gene expression in HBV-infected primary human hepatocytes.

Organism:

Hepatitis B virus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32478

96 Samples

Download data: TSV

(Submitter supplied) Background & Aims: Hepatitis B virus (HBV) infection is a major health burden worldwide and currently there is no cure. The persistence of HBV covalently closed circular DNA (cccDNA) is the major obstacle for antiviral treatment. HBV core protein (HBc) has merged as a promising antiviral target, as it plays important roles in critical steps of viral life cycle. However, whether HBc could regulate HBV cccDNA transcription remains to be illustrated. more...

Organism:

Hepatitis B virus; Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL32090

8 Samples

Download data: BW, TXT

(Submitter supplied) HBV transcription and replication increases progressively throughout postnatal liver development with maximal viral biosynthesis occurring at around four weeks of age in the HBV transgenic mouse model of chronic infection. Increasing viral biosynthesis is associated with a corresponding progressive loss of DNA methylation. The loss of DNA methylation is associated with increasing levels of 5-hydroxymethylcytosine (5hmC) residues which correlates with increased liver-enriched pioneer transcription factor Forkhead box protein A (FoxA) RNA levels, a rapid decline in postnatal liver DNA methyltransferase (Dnmt) transcripts and a very modest reduction in Ten-eleven translocation (Tet) methylcytosine dioxygenase expression. more...

Organism:

Mus musculus; Hepatitis B virus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL29102

120 Samples

Download data: FA, TXT

(Submitter supplied) Covalently closed circular DNA (cccDNA) forms the basis for replication and persistence of hepatitis B virus (HBV) in the chronically infected liver. We have previously shown through the analysis of de novo HBV infected cell lines that viral transcription is subject to regulation by posttranslational modifications (PTMs) of histone proteins bound to cccDNA. We now report the successful adaptation of this ChIPseq approach for the analysis of fine-needle patient liver biopsy specimens to investigate the role of histone PTMs in chronically HBV-infected patients. more...

Organism:

Homo sapiens; Hepatitis B virus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL15520 GPL24950

179 Samples

Download data: WIG, XLSX

(Submitter supplied) Monitor expression of all viral mRNAs including polyA-deficient viral mRNAs

Organism:

Hepatitis B virus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23794

24 Samples

Download data: GCT