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idAnoGambNW_F1_1

Organism name:
Anopheles gambiae (African malaria mosquito)
BioSample:
SAMEA12928234
BioProject:
PRJEB53260
Submitter:
WELLCOME SANGER INSTITUTE
Date:
2022/09/09
Assembly type:
haploid (principal pseudohaplotype of diploid)
Assembly level:
Chromosome
Genome representation:
full
RefSeq category:
representative genome
GenBank assembly accession:
GCA_943734735.2 (latest)
RefSeq assembly accession:
GCF_943734735.2 (latest)
RefSeq assembly and GenBank assembly identical:
yes
WGS Project:
CALSDY02
Genome coverage:
54x
Linked assembly:
GCA_943734675.1 (alternate pseudohaplotype of diploid)

IDs: 15480831 [UID] 39807018 [GenBank] 51179128 [RefSeq]

See Genome Information for Anopheles gambiae

There are 7 assemblies for this organism

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History (Show revision history)

Comment

The assembly idAnoGambNW_F1_1.2 is based on 54x PacBio HiFi data, 10X Genomics Chromium data, and Arima Hi-C data generated by the Anopheles Reference Genomes project. The assembly process included the following sequence of steps: initial PacBio assembly generation with ... hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named, ordered and oriented based on published data.  more

Global statistics

Total sequence length264,466,744
Total ungapped length264,450,744
Gaps between scaffolds0
Number of scaffolds191
Scaffold N5099,149,756
Scaffold L502
Number of contigs232
Contig N5010,625,458
Contig L508
Total number of chromosomes and plasmids4
Number of component sequences (WGS or clone)191

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Global assembly definition

Download the full sequence report
Click on the table row to see sequence details in the table to the right
Assembly Unit Name
Primary Assembly
non-nuclear
Assembly Unit: Primary Assembly (GCF_943734734.2)
Molecule nameGenBank sequenceRefSeq sequenceUnlocalized
sequences count
Chromosome XOX030909.1=NC_064600.1161
Chromosome 2OX030907.1=NC_064601.10
Chromosome 3OX030908.1=NC_064602.10
unplacedn/an/an/a26

Assembly statistics

MoleculeSequence RoleTotal
Length
Scaffold
Count
Ungapped
Length
Scaffold
N50
Spanned
Gaps
Unspanned
Gaps
AllAssembled molecule264,451,381190264,435,38199,149,756410
Chromosome XAllAssembled moleculeUnlocalized scaffolds39,617,17228,097,88911,519,283162116139,612,27228,093,98911,518,28328,097,88928,097,889100,9941192000
Chromosome 2Assembled molecule118,196,9521118,193,652118,196,95290
Chromosome 3Assembled molecule99,149,756199,144,95699,149,756150
unplacedAssembled molecule7,487,501267,484,501512,85260
MoleculeTotal
Length
Mitochondrion MT15,363