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IDs: 12718201 [UID] 33265168 [GenBank] 46319798 [RefSeq]
The assembly fAciRut3.2 is based on 61x PacBio data and Dovetail Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: HiFi reads were trio-binned using trioCanu with k-mers from ... the Illumina-sequenced parents; haplotype-specific HiFi reads were assembled separately using Hifiasm; segregation of HiC reads using Canu's 'splitHaplotype' script and k-mers from the Illumina-sequenced parents; for each haplotype assembly, haplotype-specific and unclassified HiC reads were combined to construct scaffolds using pins. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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